Homocysteine (Hcy) is a thiol-containing amino acid produced by the intracellular demethylation of methionine. Hcy is exported into plasma where it circulates mostly in its oxidized forms bound to plasma proteins. Smaller amounts of reduced homocysteine and disulfide homocystine (Hcy-SS-Hcy) are present. Total homocysteine represents the sum of all Hcy species found in plasma and serum (free plus protein-bound).
Epidemiological studies have investigated the relationship between Hcy levels in blood and cardiovascular disease (CVD). A meta analysis of 27 epidemiological studies, including more than 4000 patients, estimated that a 5 µmol/L increase in Hcy was associated with an odds ratio for coronary artery disease (CAD) of 1.6 for men and 1.8 for women, or the same that is associated with 0.5 mmol/L (20 mg/dL) increase in cholesterol. Peripheral arterial disease also showed a strong association.
Patients with chronic renal disease experience an excess morbidity and mortality due to arteriosclerotic CVD. Elevated concentration of Hcy is a frequently observed finding in the blood of these patients. Although such patients may lack some of the vitamins involved in the metabolism of Hcy, the increased levels of Hcy are mainly due to impaired removal of Hcy from the blood by the kidney.
Drugs such as methotrexate, carbamazepine, phenytoin, nitrous oxide and penicillamine interfere with the Hcy metabolism and may give elevated levels of Hcy.
Part Number: FHCY100
Description: 96-well microtire plate, liquid reagents, 6 calibrators, 2 controls
- Exclusive to Axis-Shield and approved partners
- CE-marked and FDA 510(k)-cleared
- Easy-to-use ELISA technology, compatible with automated systems for improved throughput.
- Excellent correlation with immunoassay, clinical chemistry and LC methods
- Range from 2 to 50µmol/L
- No interference from Intrinsic Factor antibodies
- No pre-analytical sample denaturation – remove a source of a variability
The Axis® Homocysteine Enzyme Immunoassay (EIA) is intended for the quantitative determination of total L-homocysteine in human serum or plasma. The device can assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria.
Axis® Homocysteine Enzyme Immunoassay (EIA) is an enzyme immunoassay for the determination of Hcy in blood. Protein-bound Hcy is reduced to free Hcy and enzymatically converted to S-adenosyl-L-homocysteine (SAH) in a separate procedure prior to the immunoassay. The enzyme is specific for the L-form of homocysteine, which is the only form present in the blood.
More information on Homocysteine can be found at www.homocysteine.co.uk
*Products may not be approved for sale in all countries. Please contact your local distributor for further details.