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Our Vision is to improve healthcare as a global provider of innovative and reliable diagnostic tests. -
Our Mission is to identify and develop novel patentable diagnostic markers and technologies which can be commercialised to meet clinical needs, both in the laboratory and at the point of care. -
We will collaborate with partners, create branded products and identify complementary businesses which support our objective of continuing to grow as a respected and profitable healthcare company. -
Our Values include meeting all current and future regulatory, quality and environmental standards appropriate for our industry and the countries in which we operate.
Enzymatic Homocysteine
As a general marker of overall health status, few tests carry greater predictive weight then Homocysteine. The amount of Homocysteine in your blood is one of the best independent indicators of how healthy you are.
Axis-Shield launched the first immunoassay for Homocysteine and we are still at the forefront of promoting this vital marker with our new Enzymatic assay for use on laboratory clinical chemistry platforms.
For more information please visit our product website at www.homocysteine.org.uk
Click the image to download our brochure
Features
- Multiple instrument applications available (Roche, BCI, Siemens and others)
- Compatible with serum, serum separator and EDTA or heparinized plasma
- Sample volume from 15µL
- Limit of detection from 0.8 µmol/L
- Assay Range up to 46 µmol/L
- Only 2 calibrators required with calibration stability up to 4 weeks
- Time to first result from 12 minutes
- Ready to use, liquid stable reagents and calibrators supplied
Intended Use
The Liquid Stable (LS) 2-Part Enzymatic Homocysteine assay is intended for in vitro quantitative determination of total homocysteine in human serum and plasma.
Assay Principle
In the Axis-Shield Enzymatic Homocysteine assay, bound or dimerised homocysteine (oxidised form) is reduced to free homocysteine, which then reacts with serine catalysed by cystathionine beta-synthase (CBS) to form L-cystathionine. Cystathionine in turn is broken down by cystathionine beta-lyase (CBL) to form homocysteine, pyruvate and ammonia. Pyruvate is then converted by lactate dehydrogenase (LDH) to lactate with NADH as coenzyme. The rate of NADH conversion to NAD is directly proportional to the concentration of homocysteine.
Performance
Precision
EDTA plasmas run in duplicate on the Roche Integra 800 over 20 days according to CLSI EP5-A2:
| Sample 1 | Sample 2 | Sample 3 | |
| Mean umol/L | 8.5 | 35.5 | 45.6 |
| Within-run CV% | 1.9% | 0.9% | 0.9% |
| Total CV% | 2.7% | 1.6% | 1.9% |
*Products may not be approved for sale in all countries. Please contact us for further details.
Axis-Shield at a Glance
A pioneering organisation focused on the development and manufacturing of important and innovative in vitro diagnostic tests for use in clinical laboratories and at the point of care.
Focus on Afinion
Axis-Shield's award winning point-of-care test system is designed for maximum simplicity coupled with laboratory accuracy and reliability for a number of key markers important for improved patient management.
